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pcdna3 1 neo vector (Addgene inc)

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Addgene inc pcdna3 1 neo vector
Pcdna3 1 Neo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2723 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 1 neo vector/product/Addgene inc
Average 96 stars, based on 2723 article reviews

pcdna3 1 neo vector - by Bioz Stars, 2026-03

96/100 stars

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p cmv neo bam p53wt (Addgene inc)

Addgene inc p cmv neo bam p53wt

A. PG13-luc reporter assay in cancer cells. HCT116 p53-null cells and U2OS-p53KO were transiently transfected with <t>p-CMV-Neo-Bam-p53wt</t> and PG13-luc together with the plasmids as indicated for 20 hours. B. PG13-luc reporter assay. U2OS-p53KO cells were transfected with the plasmids as indicated, followed with 10 μM of nutlin-3a treatment for 4 hours. C. Cell cycle profiling in HCT116 cells transiently transfected with the plasmids as indicated for 72 hours.

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A. PG13-luc reporter assay in cancer cells. HCT116 p53-null cells and U2OS-p53KO were transiently transfected with p-CMV-Neo-Bam-p53wt and PG13-luc together with the plasmids as indicated for 20 hours. B. PG13-luc reporter assay. U2OS-p53KO cells were transfected with the plasmids as indicated, followed with 10 μM of nutlin-3a treatment for 4 hours. C. Cell cycle profiling in HCT116 cells transiently transfected with the plasmids as indicated for 72 hours.

Journal: bioRxiv

Article Title: SARS-CoV-2 spike S2 subunit inhibits p53 activation of p21(WAF1), TRAIL Death Receptor DR5 and MDM2 proteins in cancer cells

doi: 10.1101/2024.04.12.589252

Figure Lengend Snippet: A. PG13-luc reporter assay in cancer cells. HCT116 p53-null cells and U2OS-p53KO were transiently transfected with p-CMV-Neo-Bam-p53wt and PG13-luc together with the plasmids as indicated for 20 hours. B. PG13-luc reporter assay. U2OS-p53KO cells were transfected with the plasmids as indicated, followed with 10 μM of nutlin-3a treatment for 4 hours. C. Cell cycle profiling in HCT116 cells transiently transfected with the plasmids as indicated for 72 hours.

Article Snippet: The plasmids pcDNA3.1-SARS2-spike (#145032) and p-CMV-Neo-Bam-p53wt (#16434) were obtained from Addgene.

Techniques: Reporter Assay, Transfection